Previous studies have shown that cationic bactericidal / permeability increasing protein (BPI) is present in both rabbit and human polymorphonuclear leukocytes are the main O2 independent bactericidal agent of these cells in several strains of Escherichia coli and Salmonella Typhimurium (1978 .. J. Biol Chem 253: 2664 - 2672, 1979 J. Biol Chem 254:. 11000 - 11009) .... To further assess the possible role of this protein in killing gram-negative bacteria, polymorphonuclear leukocytes, we measured the bactericidal activity of intact rabbit peritoneal exudate leukocytes under aerobic or anaerobic conditions and intact human leukocytes in patients with HHB. Anaerobic conditions were created by washing the cells with a stream of nitrogen. Effective removal of oxygen was demonstrated by the inability of nitrogen rozchervonile leukocytes mount a respiratory burst (measured as increase the conversion of 1 - [14C] glucose leads to S14O2 or superoxide) in bacterial mouth. In bacteria / leukocytes 10:1, killing gram-positive, resistant to BPI, epidermal staphylococcus markedly disturbed in the absence of oxygen (76 4 + / - .. 3. 3% of murders in the air space, 29 2 + / - 8 2% of murders in nitrogen) .. Almost all the increased bacterial intracellular survival. In contrast, as nonopsonized rough strain (MR-10) and opsonizovani smooth strain (MS) S. Typhimurium 395 were killed equally well in room air and nitrogen. Maximum 70 - 80 MR-10 and 30 - 40 MS killed in leukocyte or in the presence or absence of oxygen. There is no intracellular bacterial survival in conditions or that intracellular O2 independent bactericidal system (s) of rabbit polymorphonuclear leukocytes can at least meet the ingestive capacity of leukocytes. Total homogenate and crude extract acid shows a similar potential bactericidal against S. Typhimurium 395. This activity may be due to BPI content of cell fractions and virtually eliminated immune (anti-BPI), but not preimmune goat IgG-rich fractions. Opsonizatsiyi smooth MS, required for bacterial killing intact leukocytes does not affect the bacterial sensitivity to BPI in crude or purified form. Leukocytes of patients with HHB murdered inside S. Typhimurium MS 396 is almost the same as normal white blood cells. Bactericidal activity strattera no prescritpion to Escherichia coli (J5) of crude extract acid CP and normal human leukocytes was almost the same and almost completely suppressed by anti-IgG BPI-rich fractions, but not preimmune IgG-rich fractions. These data suggest that killing Gram-negative bacteria such as S. Typhimurium intact polymorphonuclear leukocytes may be due to the action of BPI. .
No comments:
Post a Comment